Learning outcomes
The aim of the lab is to guide the students through the entire creative cycle required for the design of synthetic biological systems with novel functions. The participants will go through all stages of the design-build-test process followed by analysis and interpretation of the outcome. The students acquire basic molecular biology skills and gain an experience in which they take ownership of their projects, troubleshoot their own experiments, present at frequent laboratory meetings.
Brief description of content
The course is divided into 5 subprojects. Every project will start with the in silico design of the genetic circuits to be built with the aim to gain the desired biological function. This stage will be held separately, few weeks before the laboratory stage, during the course "LTTI.00.016 Bioinformatics for Bioengineers", in order to order the synthetic DNA and other missing reagents. Each student will participate in at least three different projects. The list of the projects is subject to changes in each year depending on the progress and emerging new ideas. Four main biological systems used in synthetic biology will also be used in the course: bacteria, yeast, plants and mammalian cells.
